International Journal of Oral Research

Effect of Tricalcium Phosphate, Casein Phosphopeptide - Amorphous Calcium Phosphate and Sodium Fluoride Products on Demineralization of Artificial Advanced Enamel Lesions

Wichitra Sri-Aularawat, Siriruk Nakornchai, Sroisiri Thaweboon, Suwanna Korsuwannawong

Department of Pediatric Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand

IJOR 2012:3;e2



Objective: The objective of this study was to evaluate the demineralization improvement by calculating the demineralization areas on artificial advanced enamel lesions after treatment with tricalcium phosphate (TCP), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and sodium fluoride (NaF) products. Materials and Methods: Twenty sound extracted third molars were sectioned longitudinally at proximal surfaces to produce six sections per tooth. All sections were coated with nail varnish, leaving a window one mm long on the proximal surface and were subsequently immersed in demineralized solution for 28 days to produce lesions >300 μm in depth. All sections were randomly divided into six groups, Group A: deionized water, Group B: 0.22% NaF toothpaste, Group C: 0.21% NaF paste with TCP, Group D: 10% CPP-ACP toothmousse, Group E: 10% CPP-ACP toothmousse with 0.2% NaF and Group F: 1.1% NaF gel. The sec­tions were subjected to pH cycling for ten days. The demineralized area of each section was examined by a polarized light microscope and Image Pro Plus® software before and after treatment. The area changes were compared by Paired-T, ANOVA and LSD tests. Results: The demineralized areas had a significant difference between before and after treatment of the all groups (p = 0.00). The percent change of the demineralized areas in Group A increased by 15.09 ± 2.09%, whereas Groups B, C, D, E and F decreased by 3.62 ± 0.1%, 9.59 ± 1.78%, 4.65 ± 1.13%, 8.11 ± 1.26% and 11.31 ± 1.38%, respectively. The area changes were significantly different among groups (p < 0.05). Group A showed that the percent change had a significant increase compared with the other groups. Groups F, C, E, D and B showed that the percent change significantly decreased. Conclusion: All products can decrease demineraliza­tion on advanced enamel lesions. The 1.1% NaF gel showed the most effective in reduction of demineralization followed by 0.21% NaF paste with TCP, CPP-ACP toothmousse with 0.2% NaF, CPP-ACP toothmousse and 0.22% NaF toothpaste. 


Keywords: Demineralization, Artificial caries lesions, Tricalcium phosphate, Casein phosphopeptide-amorphous calcium phosphate, Sodium fluoride

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